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Featured Publications

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Directed evolution of engineered virus-like particles with improved

production and transduction efficiencies

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A. Raguram**; M. An; P. Z. Chen; D. R. Liu** (** denotes co-corresponding authors)

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Nat. Biotechnol. in press (2024).

eVLP manuscript

Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins.

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S. Banskota*; A. Raguram*; S. Suh; S. W. Du; J. R. Davis; E. H. Choi; X. Wang; S. C. Nielsen; G. A. Newby; P. B. Randolph;

M. J. Osborn; K. Musunuru; K. Palczewski; D. R. Liu (* denotes co-first authors)

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Cell 185, 250–265 (2022).

Delivery review Cell

Therapeutic in vivo delivery of gene editing agents.

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A. Raguram*; S. Banskota*; D. R. Liu (* denotes co-first authors)

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Cell 185, 2806–2827 (2022).

All Publications

(* denotes co-first authors, ** denotes co-corresponding authors)

22. Directed evolution of engineered virus-like particles with improved production and transduction efficiencies.

       A. Raguram**; M. An; P. Z. Chen; D. R. Liu**. Nat. Biotechnol. in press (2024). PDF | Journal link

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21. Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes in vivo.

       M. An; A. Raguram; S. W. Du; S. Banskota; J. R. Davis; G. A. Newby; P. Z. Chen; K. Palczewski; D. R. Liu. Nat. Biotechnol. 42, 1526–1537 (2024). 

       PDF | Journal link

 

20. Phage-assisted evolution and protein engineering yield compact, efficient prime editors.

       J. L. Doman*; S. Pandey*; M. E. Neugebauer; M. An; J. R. Davis; P. B. Randolph; A. McElroy; X. D. Gao; A. Raguram; M. F. Richter; K. A. Everette; S. Banskota;

       K. Tian; Y. A. Tao; J. Tolar; M. J. Osborn; D. R. Liu. Cell 186, 3983–4002 (2023). PDF | Journal link

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19. Base editing rescue of spinal muscular atrophy in cells and in mice.

       M. Arbab*; Z. Matuszek*; K. M. Kray; A. Du; G. A. Newby; A. J. Blatnik; A. Raguram; M. F. Richter; K. T. Zhao; J. M. Levy; M. W. Shen; W. D. Arnold; D. Wang;

       J. Xie; G. Gao; A. H. M. Burghes; D. R. Liu. Science 380, eadg6518 (2023). PDF | Journal link

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18. Efficient in vivo genome editing prevents hypertrophic cardiomyopathy in mice.

       D. Reichart*; G. A. Newby*; H. Wakimoto*; M. Lun; J. M. Gorham; J. J. Curran; A. Raguram; D. M. DeLaughter; D. A. Conner; J. D. C. Marsiglia; S. Kohli;

       L. Chmatal; D. C. Page; N. Zabaleta; L. Vandenberghe; D. R. Liu; J. G. Seidman; C. Seidman. Nat. Med. 29, 412–421 (2023). PDF | Journal link

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17. Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity.

       M. E. Neugebauer; A. Hsu; M. Arbab; N. A. Krasnow; A. N. McElroy; S. Pandey; J. L. Doman; T. P. Huang; A. Raguram; S. Banskota; G. A. Newby; J. Tolar;

       M. J. Osborn; D. R. Liu. Nat. Biotechnol. 41, 673–685 (2023). PDF | Journal link

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16. Precision genome editing in the eye.

       S. Suh; E. H. Choi; A. Raguram; D. R. Liu; K. Palczewski. Proc. Natl. Acad. Sci. U.S.A. 119, e2210104119 (2022). PDF | Journal link

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15. Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors.

       J. R. Davis; X. Wang; I. Witte; T. Huang; J. M. Levy; A. Raguram; S. Banskota; N. G. Seidah; K. Musunuru; D. R. Liu. Nat. Biomed. Eng. 6, 1272–1283 (2022).

       PDF | Journal link

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14. Therapeutic in vivo delivery of gene editing agents.

       A. Raguram*; S. Banskota*; D. R. Liu. Cell 185, 2806–2827 (2022). PDF | Journal link

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13. CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA.

       B. Y. Mok; A. V. Kotrys; A. Raguram; T. P. Huang; V. K. Mootha; D. R. Liu. Nat. Biotechnol. 40, 1378–1387 (2022). PDF | Journal link

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12. In vivo base editing rescues cone photoreceptors in a mouse model of early-onset inherited retinal degeneration.

       E. H. Choi*; S. Suh*; A. T. Foik; H. Leinonen; G. A. Newby; X. D. Gao; S. Banskota; T. Hoang; S. Du; Z. Dong; A. Raguram; S. Kohli; S. Blackshaw; D. C. Lyon;

       D. R. Liu; K. Palczewski. Nat. Commun. 13, 1830 (2022). PDF | Journal link

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11. Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins.

       S. Banskota*; A. Raguram*; S. Suh; S. W. Du; J. R. Davis; E. H. Choi; X. Wang; S. C. Nielsen; G. A. Newby; P. B. Randolph; M. J. Osborn; K. Musunuru;

       K. Palczewski; D. R. Liu. Cell 185, 250–265 (2022). PDF | Journal link

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10. Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing.

       A. V. Anzalone*; X. D. Gao*; C. J. Podracky*; A. T. Nelson; L. W. Koblan; A. Raguram; J. M. Levy; J. A. M. Mercer; D. R. Liu. Nat. Biotechnol. 40, 731–740 (2022).

       PDF | Journal link

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  9. Disulfide-compatible phage-assisted continuous evolution in the periplasmic space.

      M. S. Morrison; T. Wang; A. Raguram; C. Hemez; D. R. Liu. Nat. Commun. 12, 5959 (2021). PDF | Journal link

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  8. A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.

      B. Y. Mok*; M. H. de Moraes*; D. E. Bosch; A. V. Kotrys; A. Raguram; F. Hsu; M. C. Radey; S. B. Peterson; V. K. Mootha; J. D. Mougous**; D. R. Liu**.

      Nature 583, 631–637 (2020). PDF | Journal link

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  7. Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.

      J. L. Doman*; A. Raguram*; G. A. Newby; D. R. Liu. Nat. Biotechnol. 38, 620–628 (2020). PDF | Journal link

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  6. Prime genome editing in rice and wheat.

      Q. Lin*; Y. Zong*; C. Xue*; S. Wang; S. Jin; Z. Zhu; Y. Wang; A. V. Anzalone; A. Raguram; J. L. Doman; D. R. Liu; C. Gao. Nat. Biotechnol. 38, 582–585 (2020).

      PDF | Journal link

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  5. Search-and-replace genome editing without double-strand breaks or donor DNA.

      A. V. Anzalone; P. B. Randolph; J. R. Davis; A. A. Sousa; L. W. Koblan; J. M. Levy; P. J. Chen; C. Wilson; G. A. Newby; A. Raguram; D. R. Liu.

      Nature 576, 149–157 (2019). PDF | Journal link

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  4. Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.

      L. W. Koblan; J. L. Doman; C. Wilson; J. M. Levy; T. Tay; G. A. Newby; J. P. Maianti; A. Raguram; D. R. Liu. Nat. Biotechnol. 36, 843–846 (2018). PDF | Journal link

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  3. Ribozyme-catalysed RNA synthesis using triplet building blocks.

      J. Attwater; A. Raguram; A. S. Morgunov; E. Gianni; P. Holliger. eLife 7, e35255 (2018). PDF | Journal link

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  2. Rational engineering and characterization of an mAb that neutralizes Zika virus by targeting a mutationally constrained quaternary epitope.

      K. Tharakaraman*; S. Watanabe*; K. R. Chan; J. Huan; V. Subramanian; Y. H. Chionh; A. Raguram; D. Quinlan; M. McBee; E. Z. Ong; E. S. Gan; H. C. Tan; A. Tyagi;        S. Bhushan; J. Lescar; S. G. Vasudevan; E. E. Ooi**; R. Sasisekharan**. Cell Host Microbe 23, 618–627 (2018). PDF | Journal link

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  1. A chiral pentagonal polyhedral framework for characterizing virus capsid structures.

      A. Raguram; V. Sasisekharan; R. Sasisekharan. Trends Microbiol. 25, 438–446 (2017). PDF | Journal link

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